Journal:

Article Title: Functional activity of CD40 antibodies correlates to the position of binding relative to CD154

doi: 10.1046/j.1365-2567.2001.01148.x

Figure Lengend Snippet: Summary of data obtained from isotyping, affinity determination and inhibition of CD154 binding assays for the panel of anti-CD40 mAb

Article Snippet: 17 Anti-CD40 mAb producing hybridoma cell lines were expanded in Integra CL-6 growth chambers (Integra Biosciences Ltd, Letchworth, Hertfordshire, UK).

Techniques: Inhibition, Binding Assay

Journal:

Article Title: Functional activity of CD40 antibodies correlates to the position of binding relative to CD154

doi: 10.1046/j.1365-2567.2001.01148.x

Figure Lengend Snippet: FACS histogram plot illustrating inhibition of CD154-CD8 binding to CD40 transfected fibroblast cells. The filled histogram represents the positive control (i.e. cells incubated with CD154-CD8 in the absence of blocking reagents) the thin, solid line represents the negative control (i.e. cells incubated with secondary reagents only), the thick, solid line represents complete inhibition by the 10C8 mAb and the broken line indicates partial inhibition by the mAb 1G7.

Article Snippet: 17 Anti-CD40 mAb producing hybridoma cell lines were expanded in Integra CL-6 growth chambers (Integra Biosciences Ltd, Letchworth, Hertfordshire, UK).

Techniques: Inhibition, Binding Assay, Transfection, Positive Control, Incubation, Blocking Assay, Negative Control